Electrochemical detection of miRNAs
نویسندگان
چکیده
The recent progress made in the development of electrochemical methods for microRNA (miRNA) detection is presented. This progress is conceived to be largely due to the invention of novel assay methodologies and the use of various bioreagents and nanostructures. These enable a rigorous control over the sensing interface, provide enormous signal amplifications and single-base mismatch specificity. Femtomolar or even subfemtomolar detection limits were shown to be feasible by electrochemical assays. Thus electrochemical detection methodologies are of perspective for diagnostic miRNA detection.
منابع مشابه
Electrochemical miRNA Biosensors: The Benefits of Nanotechnology
The importance of nanotechnology in medical technologies, especially biomedical diagnostics, is indubitable. By taking advantages of nanomaterials, many medical diagnostics methods have been developed so far, including electrochemical nanobiosensors. They have been used for quantification of different clinical biomarkers for detecting, screening, or follow up a disease. microRNAs (miRNAs) are o...
متن کاملNanomaterials-Based Sensing Strategies for Electrochemical Detection of MicroRNAs
MicroRNAs (miRNAs) play important functions in post-transcriptional regulation of gene expression. They have been regarded as reliable molecular biomarkers for many diseases including cancer. However, the content of miRNAs in cells can be low down to a few molecules per cell. Thus, highly sensitive analytical methods for miRNAs detection are desired. Recently, electrochemical biosensors have he...
متن کاملElectron transfer mediated electrochemical biosensor for microRNAs detection based on metal ion functionalized titanium phosphate nanospheres at attomole level.
MicroRNAs (miRNAs) have emerged as new candidates as diagnostic and prognostic biomarkers for the detection of a wide variety of cancers; thus, sensitive and selective detection of microRNAs is significant for early-phase cancer diagnosis and disease prevention. A novel and simple electrochemical miRNA biosensor was developed using Cd(2+)-modified titanium phosphate nanoparticles as signal unit...
متن کاملSensitive detection of microRNAs based on the conversion of colorimetric assay into electrochemical analysis with duplex-specific nuclease-assisted signal amplification
miRNAs have emerged as new biomarkers for the detection of a wide variety of cancers. By employing duplex-specific nuclease for signal amplification and gold nanoparticles (AuNPs) as the carriers of detection probes, a novel electrochemical assay of miRNAs was performed. The method is based on conversion of the well-known colorimetric assay into electrochemical analysis with enhanced sensitivit...
متن کاملCorrigendum: Highly sensitive dual mode electrochemical platform for microRNA detection
MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of ...
متن کامل